9OKZ

16mer self-complementary duplex RNA with dG:s(2)C pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2025-04-17
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.00003
Spacegroup name	P 31
Unit cell lengths	42.502, 42.502, 122.713
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.808 - 1.919
Rwork	0.187
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	2.058
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.919	1.919
Rmerge	0.044	0.570
Rmeas	0.049	0.638
Rpim	0.021	0.284
Number of reflections	18877	919
<I/σ(I)>	32.8	2.8
Completeness [%]	99.3	99.4
Redundancy	5	4.9
CC(1/2)	1.000	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES pH 6.5, 0.06 M Manganese (II) chloride, 15.00 % w/v Polyethylene glycol 20,000