9OKW

16mer self-complementary duplex RNA with dG:C pair sequence 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-06-28
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97648
Spacegroup name	H 3 2
Unit cell lengths	41.258, 41.258, 124.595
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.530 - 1.540
R-factor	0.2036
Rwork	0.199
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.484
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.570
High resolution limit [Å]	1.540	1.540
Rmerge	0.087	0.335
Rmeas	0.093	0.373
Rpim	0.032	0.169
Number of reflections	6327	294
<I/σ(I)>	24.5	3.8
Completeness [%]	99.9	100
Redundancy	8.5	4.9
CC(1/2)	0.974	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	25 % v/v Polyethylene glycol monomethyl ether 550, 50 mM HEPES pH 7.0, 10 mM Magnesium chloride