9OKV

16mer self-complementary duplex RNA with dA:s(2)U pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-10-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	H 3 2
Unit cell lengths	41.147, 41.147, 124.373
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.458 - 1.233
Rwork	0.183
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.044
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.250
High resolution limit [Å]	1.230	1.230
Rmerge	0.040	0.214
Rmeas	0.043	0.251
Rpim	0.014	0.127
Number of reflections	11714	388
<I/σ(I)>	52	5.2
Completeness [%]	96.2	62.3
Redundancy	8.3	3.4
CC(1/2)	1.000	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.02 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% v/v (+/-)-2-Methyl-2,4-pentanediol