9OJJ

sx20S complex (NSF-alphaSNAP-syntaxin-1a), 4:4 alphaSNAP-syntaxin-1a subcomplex local refinement, non-hydrolyzing, class 1

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	4.07
Number of Particle	975203
Software	RELION,PHENIX,cryoSPARC,SerialEM,CTFFIND,Coot,UCSF ChimeraX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION