9OIM

The von Hippel Lindau-ElonginB-ElonginC (VCB) complex with fragment 9

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-04
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.000
Spacegroup name	P 41 2 2
Unit cell lengths	93.386, 93.386, 362.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.830 - 2.610
R-factor	0.2278
Rwork	0.225
R-free	0.28330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.223
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.830	2.700
High resolution limit [Å]	2.610	2.610
Rmerge	0.072	1.010
Rpim	0.072	1.010
Number of reflections	88336	47542
<I/σ(I)>	10.6	0.7
Completeness [%]	95.8	97
Redundancy	1.9
CC(1/2)	0.994	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium cacodylate pH 5.5, 0.2 M magnesium acetate, 5 mM DTT and 14-20% PEG8000