9OIK

Structure of S. Typhimurium 14028 Gifsy-1 prophage HepS bound to bacteriophage lambda J Tail Tip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97935
Spacegroup name	C 1 2 1
Unit cell lengths	78.190, 93.878, 92.456
Unit cell angles	90.00, 110.81, 90.00

Refinement procedure

Resolution	43.210 - 1.857
R-factor	0.1842
Rwork	0.183
R-free	0.20930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.808
Data reduction software	XDS (Jun 30, 2024 (BUILT 20241002))
Data scaling software	autoPROC (1.0.5 (20240123))
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.210	1.889
High resolution limit [Å]	1.857	1.857
Rmerge		1.040
Rpim		0.547
Number of reflections	102985	50821
<I/σ(I)>	6.7	1.7
Completeness [%]	100.0
Redundancy	4.4
CC(1/2)	0.996	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Sodium Acetate, 16% PEG 4000, 0.1 M Tris pH 8.5