9OIJ

Structure of S. Typhimurium 14028 Gifsy-1 prophage HepS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 32 2 1
Unit cell lengths	130.717, 130.717, 44.797
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.390 - 2.050
R-factor	0.2491
Rwork	0.246
R-free	0.29680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.461
Data reduction software	XDS (Jun 30, 2023 (BUILT 20230630))
Data scaling software	autoPROC (1.0.5 (20211020))
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.690	2.063
High resolution limit [Å]	2.028	2.028
Rpim	0.032	1.667
Number of reflections	27169	27169
<I/σ(I)>	12.2	0.4
Completeness [%]	100.0
Redundancy	20.7
CC(1/2)		0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M CaCl2, 0.1 M Tris pH 8.5, 15% (w/v) PEG 4000