9OGO

Crystal Structure of KPT185 in complex with CRM1(EH mutant)-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2016-11-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.077, 106.077, 303.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.240 - 2.370
R-factor	0.194
Rwork	0.193
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.461
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.410
High resolution limit [Å]	2.370	6.430	2.370
Rmerge	0.089	0.022	1.000
Rmeas		0.023
Rpim	0.034	0.007	0.468
Number of reflections	71232	3938	3485
<I/σ(I)>	19.4
Completeness [%]	99.8	99.9	99.3
Redundancy	8.7	11	8.1
CC(1/2)		0.999	0.509

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	298	17% PEG3350, 100 mM Bis-Tris (pH 6.6) and 200 mM ammonium nitrate