9OB8

Crystal structure of human fatty acid binding protein 4 (FABP4) bound to 8-anilino-1-naphthalenesulfonic acid (ANS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-16
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	32.837, 53.744, 74.844
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.680 - 1.400
R-factor	0.1867
Rwork	0.186
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.794
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.240	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.056	0.952
Rpim	0.027	0.477
Number of reflections	26876	1314
<I/σ(I)>	72.1
Completeness [%]	99.8
Redundancy	11
CC(1/2)		0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1.6 M sodium citrate or 30% PEG4000, 0.2 M lithium sulfate, 0.1 M Tris-HCl