9OAQ

Crystal structure of antibody Fab G001-59 from IAVI G001 human trial in complex with a germline-targeting gp120 engineered outer domain eOD-GT8-mingly-N276

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	102.685, 133.973, 154.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.500 - 2.540
R-factor	0.2094
Rwork	0.208
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.538
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.540	2.540
Number of reflections	34890	1696
<I/σ(I)>	14.5
Completeness [%]	99.0
Redundancy	11.4
CC(1/2)	0.997	0.393

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	in 20% (w/v) PEG 3350 and 0.2 M magnesium sulfate at pH 5.9