9OA1
Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:5 stoichiometry ratio.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.66 |
| Number of Particle | 7250518 |
| Software | PHENIX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
