9O8B

[5,8,6,P-2] Shifted tensegrity triangle with an (arm, center, arm) distribution of (5, 8, 6) base pairs, 2 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.991870
Spacegroup name	P 63
Unit cell lengths	131.382, 131.382, 52.061
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.000 - 7.440
R-factor	0.2066
Rwork	0.198
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.883
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.890	56.890	9.040
High resolution limit [Å]	7.440	11.527	7.442
Number of reflections	628	208	208
<I/σ(I)>	11.8
Completeness [%]	88.7
Redundancy	13.7
CC(1/2)	0.999	0.999	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate