9O7V

Crystal Structure of the RIb:C Heterodimer of PKA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-14
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	103.497, 103.497, 313.382
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.371 - 3.700
R-factor	0.2343
Rwork	0.231
R-free	0.29310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.052
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.780
High resolution limit [Å]	3.700	3.700
Rmerge	0.069	0.470
Number of reflections	11338	638
<I/σ(I)>	30.8	8.4
Completeness [%]	99.5	99
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	1.4M ammonium sulfate and 0.1M sodium acetate at pH 5.3.