9O6O

Structure of SigLec-10 in complex with 2,6-Sialyllactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 65 2 2
Unit cell lengths	83.188, 83.188, 484.668
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.370 - 2.700
R-factor	0.22356
Rwork	0.221
R-free	0.27626
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.763
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.110	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.192	0.590
Rmeas	0.199	0.612
Rpim	0.049	0.160
Total number of observations	474434	49842
Number of reflections	28605	3614
<I/σ(I)>	12.6	3.1
Completeness [%]	99.8
Redundancy	16.6	13.8
CC(1/2)	0.996	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.085 M Sodium Citrate pH 5.1, 0.17 M Ammonium Acetate, 15% v/v Glycerol, and 29% w/v PEG 4000