9O6H

The Structure of PRMT4 in complex with SGC8172

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9774
Spacegroup name	P 21 21 2
Unit cell lengths	74.670, 98.984, 206.715
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.280 - 1.970
R-factor	0.2331
Rwork	0.232
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.640
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.320	2.000
High resolution limit [Å]	1.970	1.970
Rmerge	0.235	2.052
Rmeas	0.256	2.230
Rpim	0.099	0.865
Total number of observations	717838	34786
Number of reflections	109179	5366
<I/σ(I)>	4.4	0.3
Completeness [%]	100.0
Redundancy	6.6	6.5
CC(1/2)	0.983	0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium tartrate dibasic, 20% (w/v) PEG 3,350