9O66
Crystal Structure of Tryptophanyl-tRNA synthetase from Klebsiella aerogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 19-ID |
Synchrotron site | NSLS-II |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-12-15 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 0.9786 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.156, 75.510, 56.754 |
Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
Resolution | 43.270 - 2.100 |
R-factor | 0.2036 |
Rwork | 0.201 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.544 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_5660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.770 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.173 | 1.098 |
Rmeas | 0.188 | 1.191 |
Rpim | 0.073 | 0.456 |
Total number of observations | 251613 | 20511 |
Number of reflections | 37570 | 3047 |
<I/σ(I)> | 8.8 | 2.3 |
Completeness [%] | 99.8 | |
Redundancy | 6.7 | 6.7 |
CC(1/2) | 0.978 | 0.618 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Morpheus A1: 20%(v/v) PEG 500 MME, 10%(w/v) PEG 20000, 100 mM Imidazole/MES, pH 6.5, 30 mM MgCl2 and 30 mM CaCl2. KlaeA.00241.a.UX1.PW39324 at 11.1 mg/mL. plate 19612 A1 drop 1. Puck: PSL-1906, Cryo: Direct |