9O4X
Gamma delta T cell receptor bound to CD1d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 178.093, 178.093, 75.428 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.650 - 2.860 |
| R-factor | 0.2177 |
| Rwork | 0.215 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.943 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.650 | 3.020 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rpim | 0.491 | |
| Number of reflections | 28516 | 4072 |
| <I/σ(I)> | 16.4 | 2.3 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 10.4 | 10.6 |
| CC(1/2) | 0.999 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 3350, 0.05M CBTP pH 5 |
