9O4D
GutD in complex with guanine and cWW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-29 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.292, 101.319, 121.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.180 - 2.300 |
| R-factor | 0.2007 |
| Rwork | 0.199 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.781 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.180 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.053 | 0.187 |
| Rmeas | 0.075 | |
| Number of reflections | 36397 | 3568 |
| <I/σ(I)> | 3.03 | |
| Completeness [%] | 97.0 | 96.33 |
| Redundancy | 2 | |
| CC(1/2) | 0.995 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M HEPES 30%PEG400 0.1M magnesium chloride |
