9O47

RNA dodecamer containing a serinol nucleic acid.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2+ 70 kV
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-05
Detector	Bruker PHOTON III
Wavelength(s)	1.342
Spacegroup name	P 1
Unit cell lengths	24.021, 29.185, 29.782
Unit cell angles	110.87, 100.29, 111.58

Refinement procedure

Resolution	16.240 - 1.600
R-factor	0.1972
Rwork	0.196
R-free	0.22330
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.936
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	HKL2Map
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.240	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	8629	2924
<I/σ(I)>	3.4
Completeness [%]	98.3
Redundancy	2.9
CC(1/2)	0.997	0.259

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293.15	Drops contained 400 nanoliters of 1 mM RNA and 400 nanoliters of buffer containing 10% 2-methyl-2,4- pentanediol (MPD), 12 mM spermine 4 HCl, 80 mM potassium chloride, and 40 mM sodium cacodylate, pH 6.0. Drops were equilibrated against reservoirs containing 70 microliters of 40% MPD