9O3J

Crystal structure of the wild-type drug-free Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.60A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	208.560, 449.050, 616.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	123.940 - 2.600
R-factor	0.2318
Rwork	0.229
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvk
RMSD bond length	0.004
RMSD bond angle	0.856
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	123.940	123.940	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.222	0.065	2.249
Rmeas	0.241	0.070	2.446
Total number of observations	11517417
Number of reflections	1747233	20434	128675
<I/σ(I)>	7.05	22.21	0.82
Completeness [%]	99.9	99	99.9
Redundancy	6.592	6.86	6.33
CC(1/2)	0.990	0.992	0.137

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME