9O3I

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ketolide telithromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.80A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	210.040, 447.590, 618.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	124.580 - 2.800
R-factor	0.2314
Rwork	0.229
R-free	0.28090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvk
RMSD bond length	0.004
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	124.580	124.580	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.306	0.064	2.397
Rmeas	0.335	0.069	2.631
Total number of observations	8733245
Number of reflections	1401935	16385	103054
<I/σ(I)>	7.28	29.47	0.88
Completeness [%]	99.2	97.8	99.1
Redundancy	6.229	6.925	6.048
CC(1/2)	0.982	0.994	0.120

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME