9O3H

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.65A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.260, 449.450, 619.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	124.440 - 2.650
R-factor	0.2273
Rwork	0.225
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvk
RMSD bond length	0.004
RMSD bond angle	0.846
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	124.440	124.440	2.720
High resolution limit [Å]	2.650	11.850	2.650
Rmerge	0.229	0.056	1.945
Rmeas	0.247	0.061	2.102
Total number of observations	11273775
Number of reflections	1653983	19353	121316
<I/σ(I)>	7.41	23.43	0.96
Completeness [%]	99.3	98.3	98.9
Redundancy	6.816	6.812	6.862
CC(1/2)	0.991	0.997	0.163

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME