9O37

The structure of PRMT4 in complex with YD1305

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-08
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 32 2 1
Unit cell lengths	84.719, 84.719, 130.194
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.690 - 2.110
R-factor	0.1995
Rwork	0.197
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.919
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.100	2.150
High resolution limit [Å]	2.110	2.110
Rmerge	0.308	2.703
Rmeas	0.325	2.847
Rpim	0.103	0.889
Total number of observations	312310	15710
Number of reflections	31792	1549
<I/σ(I)>	7.2	0.7
Completeness [%]	100.0
Redundancy	9.8	10.1
CC(1/2)	0.967	0.285

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M calcium acetate hydrate, 0.1M HEPES: NaOH, pH 7.5, 10% (w/v) PEG 8,000