9O2M

Heparanase P6 in complex with fragment C5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	59.561, 75.849, 124.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.200 - 2.000
R-factor	0.1638
Rwork	0.162
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.795
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	2.151
Rmeas	2.273	21.968
Rpim	0.683	6.666
Number of reflections	48101	2703
<I/σ(I)>	4.8
Completeness [%]	98.6
Redundancy	13.2	13.2
CC(1/2)	0.488	0.486

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Ammonium sulfate, 0.1 M ammoniuma acetate, 25% PEG 4000