9O2J

Heparanase P6 in complex with fragment C5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	58.465, 75.192, 123.153
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.150 - 2.150
R-factor	0.2165
Rwork	0.214
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.964
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.150	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.829
Rmeas	0.860	49.092
Rpim	0.230	12.940
Total number of observations	411599
Number of reflections	30322	2595
<I/σ(I)>	6.3
Completeness [%]	100.0
Redundancy	13.6	14.1
CC(1/2)	0.990	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Ammonium sulfate, 0.1 M ammoniuma acetate, 25% PEG 4000