9O2H

Heparanase P6 in complex with fragment J92

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	58.378, 74.035, 123.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.520 - 1.750
R-factor	0.1849
Rwork	0.184
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.520	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.252
Rmeas	0.262	11.527
Rpim	0.071	3.052
Total number of observations	746049
Number of reflections	55008	2974
<I/σ(I)>	8.8
Completeness [%]	100.0
Redundancy	13.6	13.9
CC(1/2)	0.998	0.265

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Ammonium sulfate, 0.1 M ammoniuma acetate, 25% PEG 4000