9O1L
TMEM16F in liposomes in the absence of Ca2+ (expanded state)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.91 |
| Number of Particle | 4552656 |
| Software | Topaz,cryoSPARC,Leginon,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
