9NZY

Structure of Methanogen MtxX (Methanogen Marker Protein MMP4) from Methanothermobacter thermautotrophicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953653
Spacegroup name	P 1 21 1
Unit cell lengths	56.484, 72.310, 65.580
Unit cell angles	90.00, 96.11, 90.00

Refinement procedure

Resolution	65.290 - 1.600
Rwork	0.163
R-free	0.18500
Structure solution method	SIRAS
RMSD bond length	0.010
RMSD bond angle	1.721
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.100))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.290	1.630
High resolution limit [Å]	1.600	1.600
Rmeas	0.071	1.000
Rpim	0.027	0.480
Number of reflections	69258	3397
<I/σ(I)>	15.4	1
Completeness [%]	100.0	99.6
Redundancy	6.3	4.3
CC(1/2)	1.000	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	297	0.1 M Na HEPES pH 7.5, 10% w/v PEG 8000, 8% ethylene glycol