9NZL

Crystal structure of Yck2 with DJS-02-24-06

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-12
Detector	DECTRIS EIGER R 1M
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	91.824, 91.824, 118.714
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.970 - 1.920
R-factor	0.1605
Rwork	0.158
R-free	0.19970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.727
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.970	1.990
High resolution limit [Å]	1.920	1.920
Rmerge	0.080	0.469
Number of reflections	28502	2849
<I/σ(I)>	12.67	1.67
Completeness [%]	100.0	99.8
Redundancy	4.2	2.7
CC(1/2)	0.999	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M Tris pH 8, 25 mM Magnesium chloride, 20% (w/v) PEG3350