9NZI

NONO homodimer bound to (R)-SKBG-1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	96.627, 71.141, 101.748
Unit cell angles	90.00, 118.66, 90.00

Refinement procedure

Resolution	48.310 - 2.520
R-factor	0.2274
Rwork	0.225
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.399
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	MOLREP (11.7.03)
Refinement software	PHENIX (1.21rc1_5156)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.310	2.630
High resolution limit [Å]	2.520	2.520
Rmerge	0.045	1.046
Rmeas	0.062	1.232
Rpim	0.033	0.646
Number of reflections	40730	3954
<I/σ(I)>	11.6	1.2
Completeness [%]	99.3	97.6
Redundancy	3.5	3.5
CC(1/2)	0.998	0.475

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Sodium HEPES pH 8 26% (w/v) PEG 2000 MME 1:1 drop:reservoir cryprotected with 20% glycerol