9NZH

Crystal Structure of Amylin-NHO-18 binder complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92017
Spacegroup name	P 32 2 1
Unit cell lengths	59.189, 59.189, 90.946
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.020 - 2.030
R-factor	0.1895
Rwork	0.184
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.664
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.020	2.140
High resolution limit [Å]	2.030	2.030
Rmerge	0.246	1.265
Rpim	0.109	0.613
Number of reflections	12402	1771
<I/σ(I)>	5.1	1.6
Completeness [%]	100.0	100
Redundancy	6.6	5.9
CC(1/2)	0.984	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	3.2 M Ammonium sulfate, 0.1 M Citrate pH 5.0