9NZF

Crystal structure of Fab MAM01 in complex with minor repeat region peptide from circumsporozoite protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-18
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	43.459, 66.337, 83.990
Unit cell angles	90.00, 96.06, 90.00

Refinement procedure

Resolution	28.640 - 1.840
R-factor	0.192
Rwork	0.190
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.917
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.910
High resolution limit [Å]	1.840	1.840
Rmerge	0.097	0.657
Rpim	0.066
Number of reflections	37764	1967
<I/σ(I)>	16.5	1.67
Completeness [%]	92.8
Redundancy	3
CC(1/2)	0.988	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M potassium acetate, pH 7.8 and 20% (w/v) polyethylene glycol 3350