9NZ9

Crystal structure of product-bound human OGG1(WT)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.1158
Spacegroup name	P 65 2 2
Unit cell lengths	91.543, 91.543, 213.389
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.320 - 2.000
R-factor	0.1882
Rwork	0.185
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.002
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	213.390	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.125
Number of reflections	36687	2592
<I/σ(I)>	14
Completeness [%]	99.9	98.3
Redundancy	19.1
CC(1/2)	0.998	0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	288	50 mM MES, pH 6.0, 16-23% PEG6000, cryoprotectant: 25% ethylene glycol