9NZ7
Crystal structure of human DYNLL1-S64A/S88A mutant dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-22 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.963, 38.600, 44.933 |
| Unit cell angles | 90.00, 100.30, 90.00 |
Refinement procedure
| Resolution | 40.080 - 1.410 |
| R-factor | 0.2042 |
| Rwork | 0.203 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.821 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.084 | 1.432 |
| High resolution limit [Å] | 1.408 | 1.408 |
| Rmerge | 0.069 | 1.105 |
| Rmeas | 0.077 | |
| Number of reflections | 47933 | 1788 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 89.4 | 67.2 |
| Redundancy | 4.9 | |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 24% PEG2000 MME, 50 mM calcium chloride, 100 mM HEPES, 10 mM DTT, 10% glycerol |
