9NY8

Crystal structure of the ribose operon repressor, RbsR, bound to ribose operon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978560
Spacegroup name	P 31 2 1
Unit cell lengths	125.530, 125.530, 122.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.840 - 2.100
R-factor	0.1937
Rwork	0.192
R-free	0.22327
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.058
Data reduction software	xia2
Data scaling software	STARANISO
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.840	2.330
High resolution limit [Å]	2.100	2.100
Rpim	0.045
Number of reflections	47896	2397
<I/σ(I)>	15.7	1.3
Completeness [%]	94.1
Redundancy	30.5
CC(1/2)	0.999	0.184

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	0.2 M sodium sulfate, 0.1 M bis-tris-propane (pH 6.5), and 20% PEG 3350