9NY7
Crystal structure of the ribose operon repressor, RbsR, bound to ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979493 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.461, 87.353, 130.942 |
| Unit cell angles | 90.00, 100.38, 90.00 |
Refinement procedure
| Resolution | 33.820 - 2.070 |
| R-factor | 0.19361 |
| Rwork | 0.191 |
| R-free | 0.23438 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.247 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.820 | 2.367 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rpim | 0.118 | |
| Number of reflections | 72942 | 3646 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 92.1 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.985 | 0.573 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293.15 | 0.2 M sodium malonate (pH 4.0) and 20% PEG 3350 |
