9NXH
An alpha-l-arabinofuranosidase (AtAbf43C) from Acetivibrio thermocellus DSM1313 bound to arabinofuranose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.938, 81.046, 76.920 |
| Unit cell angles | 90.00, 119.91, 90.00 |
Refinement procedure
| Resolution | 29.600 - 1.750 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.155 | 0.807 |
| Rmeas | 0.168 | 0.875 |
| Rpim | 0.063 | 0.335 |
| Total number of observations | 324311 | 45244 |
| Number of reflections | 46612 | 6764 |
| <I/σ(I)> | 8.4 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 7 | 6.7 |
| CC(1/2) | 0.996 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293 | 2-propanol, PEG3350, HEPES pH 7.7, AmSO4, Glycerol, Arabinose |
