9NXG
An alpha-l-arabinofuranosidase (AtAbf43C) from Acetivibrio thermocellus DSM1313
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92015 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.265, 81.614, 82.105 |
| Unit cell angles | 90.00, 108.40, 90.00 |
Refinement procedure
| Resolution | 28.930 - 1.320 |
| R-factor | 0.1584 |
| Rwork | 0.158 |
| R-free | 0.17230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.874 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17_3644: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.930 | 1.350 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.046 | 0.359 |
| Rmeas | 0.054 | 0.452 |
| Rpim | 0.029 | 0.271 |
| Total number of observations | 783892 | 40776 |
| Number of reflections | 232133 | 15790 |
| <I/σ(I)> | 16.7 | 2.7 |
| Completeness [%] | 98.9 | |
| Redundancy | 3.4 | 2.6 |
| CC(1/2) | 0.999 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293 | 2-Propanol, PEG3350, HEPES pH 7.7, AmSO4 |
