9NTI
Chimeric Adenosine deaminase growth factor (ADGF) apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.444, 109.268, 173.275 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.210 - 3.000 |
| R-factor | 0.2202 |
| Rwork | 0.216 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.676 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.210 | 3.100 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rpim | 0.121 | 0.630 |
| Number of reflections | 519122 | 2644 |
| <I/σ(I)> | 7 | 1 |
| Completeness [%] | 99.3 | |
| Redundancy | 19.2 | |
| CC(1/2) | 0.985 | 0.513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium acetate trihydrate pH 4.5, 25% w/v Polyethylene glycol 3,350 |
