9NTE

Helix pomatia AMP deaminase (HPAMPD) apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	76.229, 82.429, 211.423
Unit cell angles	90.00, 92.21, 90.00

Refinement procedure

Resolution	19.950 - 1.750
R-factor	0.1644
Rwork	0.163
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	1.780
High resolution limit [Å]	1.750	1.750
Rpim	0.075	0.681
Number of reflections	263020	13005
<I/σ(I)>	6.5	1.1
Completeness [%]	99.8
Redundancy	4.4
CC(1/2)	0.978	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000