9NSF
Crystal Structure of the Complex of Side-VI IG1 Domain and Beat-Vc IG1+2 Domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2015-02-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 61 |
| Unit cell lengths | 87.480, 87.480, 387.379 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.140 - 3.930 |
| R-factor | 0.2363 |
| Rwork | 0.234 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.771 |
| Data reduction software | XDS (20241002) |
| Data scaling software | STARANISO (3.351) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (dev_5533) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.200 |
| High resolution limit [Å] | 3.930 | 3.930 |
| Rmerge | 0.238 | 0.500 |
| Rmeas | 0.256 | 0.538 |
| Number of reflections | 9652 | 690 |
| <I/σ(I)> | 5.99 | 3.82 |
| Completeness [%] | 64.9 | 26 |
| Redundancy | 7.6 | 7.3 |
| CC(1/2) | 0.991 | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1.3 M Potassium/Sodium tartrate, 0.1 M HEPES pH 8.0 |
