9NS8

Crystal Structure of Side-VI IG1 Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	120
Detector technology	PIXEL
Collection date	2015-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	72.770, 181.420, 300.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.510 - 2.510
R-factor	0.209
Rwork	0.208
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.729
Data reduction software	XDS (March 1, 2015)
Data scaling software	XDS (March 1, 2015)
Phasing software	MOLREP (11.5.04)
Refinement software	PHENIX (2.0rc1_5617)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.510	2.510
Rmerge	0.104	0.112
Rmeas	0.113	0.129
Number of reflections	66928	3689
<I/σ(I)>	15.66	1.22
Completeness [%]	97.8	73.8
Redundancy	6.3	3
CC(1/2)	0.998	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	1.8 M Sodium acetate trihydrate pH 7.0, 0.1 M Bis-tris propane pH 7.0