9NS4
Structure of S. pombe A-Adding Enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.656, 115.906, 84.955 |
| Unit cell angles | 90.00, 128.93, 90.00 |
Refinement procedure
| Resolution | 37.210 - 2.100 |
| R-factor | 0.2107 |
| Rwork | 0.210 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.556 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 40926 | 22413 |
| <I/σ(I)> | 14.34 | 1.09 |
| Completeness [%] | 96.7 | 95.7 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.999 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | Protein solution (CCA2+tRNACC minihelix) in 20 mM Tris pH 7.5, 100 mM NaCl) was mixed 1:0.7:0.4 with well solution (100 mM sodium acetate pH 4.6, 1.8 M ammonium sulfate) and crystal seed stock. Cyro condition was well solution with 25% ethylene glycol added |
