9NRV

Crystal structure of the H5 influenza virus hemagglutinin from A/duck/France/161108h/2016 (H5N8) clade 2.3.4.4b in complex with avian receptor analog LSTa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	94.958, 176.980, 224.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.970 - 2.890
R-factor	0.1796
Rwork	0.178
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.022
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5127: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	2.970
High resolution limit [Å]	2.890	2.890
Rpim	0.120	0.800
Number of reflections	85027	5581
<I/σ(I)>	5.5	0.7
Completeness [%]	99.9
Redundancy	6.1
CC(1/2)	0.968	0.402

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M sodium citrate, pH 6.5