9NQT

[3,5,11-2] Shifted DNA assembly with an aspect ratio of (3,5,11) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00001
Spacegroup name	P 32
Unit cell lengths	68.521, 68.521, 59.634
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.260 - 6.090
R-factor	0.1393
Rwork	0.138
R-free	0.15560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.570
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.341	59.341	7.432
High resolution limit [Å]	6.090	9.393	6.090
Number of reflections	612	204	204
<I/σ(I)>	7
Completeness [%]	86.7
Redundancy	10.4
CC(1/2)	0.996	0.996	0.105

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate