9NQ2
Crystal Structure of Side-IV IG1 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2017-03-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.544, 29.766, 41.969 |
| Unit cell angles | 90.00, 91.43, 90.00 |
Refinement procedure
| Resolution | 47.260 - 2.400 |
| R-factor | 0.1917 |
| Rwork | 0.188 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.727 |
| Data reduction software | XDS |
| Data scaling software | XDS (20161205) |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.083 | 0.254 |
| Rmeas | 0.115 | 0.350 |
| Number of reflections | 7797 | 1196 |
| <I/σ(I)> | 7.87 | 3.4 |
| Completeness [%] | 86.6 | 81.7 |
| Redundancy | 1.6 | 1.5 |
| CC(1/2) | 0.987 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M Ammonium sulfate, 15% (w/v) PEG 4000, 17% Glycerol |
