9NPV

[4,8,7-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (4,8,7) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00743
Spacegroup name	H 3
Unit cell lengths	110.467, 110.467, 66.856
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.890 - 3.000
R-factor	0.1889
Rwork	0.187
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.666
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.234	55.234	3.380
High resolution limit [Å]	2.998	9.334	2.998
Number of reflections	3444	204	202
<I/σ(I)>	11.5
Completeness [%]	74.7
Redundancy	10.7
CC(1/2)	0.999	0.999	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate