9NOD

[4,3,12-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (4,3,12) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979338
Spacegroup name	H 3
Unit cell lengths	89.487, 89.487, 139.818
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.340 - 7.230
R-factor	0.2411
Rwork	0.232
R-free	0.26160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.696
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.782	67.782	10.032
High resolution limit [Å]	7.230	10.084	7.230
Number of reflections	446	223	223
<I/σ(I)>	4.3
Completeness [%]	80.7
Redundancy	5.1
CC(1/2)	0.996	0.996	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate