9NM5
Crystal structure of human glutamine synthetase in complex with ADP and phosphinothricin phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.398, 130.981, 191.624 |
| Unit cell angles | 90.00, 101.41, 90.00 |
Refinement procedure
| Resolution | 37.540 - 1.850 |
| R-factor | 0.1322 |
| Rwork | 0.131 |
| R-free | 0.15940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.754 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_5484: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.180 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.112 | 0.584 |
| Rmeas | 0.134 | 0.698 |
| Rpim | 0.073 | 0.378 |
| Total number of observations | 1236134 | 61119 |
| Number of reflections | 374524 | 18657 |
| <I/σ(I)> | 6.9 | 1.8 |
| Completeness [%] | 99.4 | |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.992 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 35% (v/v) 2-methyl-2,4-pentanediol, 100 mM sodium acetate/acetic acid pH 4.5 |
