9NLT

Crystal Structure of Flpp3-minibinder687 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	36.166, 42.891, 54.051
Unit cell angles	90.00, 96.28, 90.00

Refinement procedure

Resolution	35.950 - 2.370
R-factor	0.2264
Rwork	0.222
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.447
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.950	2.500
High resolution limit [Å]	2.370	2.370
Rmerge	0.327	1.475
Rpim	0.212	0.663
Number of reflections	6471	969
<I/σ(I)>	4.3	1.9
Completeness [%]	95.0	99.9
Redundancy	6.3	6.5
CC(1/2)	0.977	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M Bis Tris Propane pH 6.5, 0.2 M Sodium iodide, 20 % w/v PEG 3350 and 10 % v/v Ethylene glycol